![]() ![]() Performing the Soxhlet method of ethanol extractionĪllowing the students to carry out this section of the investigation provides an added layer to what would be a standard microbiology assay. As well as supporting scientific thinking, laboratory skill and competency, this activity allows students to partake in an investigation with cross-discipline approaches to the very relevant and current healthcare issue of antimicrobials. This article presents information ensuring the existing extraction method is achievable in the teaching laboratory, while allowing students hands-on investigative experience. ![]() The aim of this activity is to investigate the antimicrobial effects of plant material after extracting compounds using the relatively simple Soxhlet method ( 6). This article describes a procedure for retrieving the antimicrobial compounds (essential oils) from the plant thyme ( Thymus vulgaris) and testing it against a variety of microorganisms, both Gram-negative and Gram-positive, for antimicrobial effect ( 4). As the issue of antimicrobial resistance continues to grow, there is a renewed interest in deriving antimicrobial products from natural compounds, particularly extracts from plant material ( 8). Investigating the effects of antimicrobial products on microorganisms is a common procedure carried out in many microbiology laboratory courses, often using antibiotics or disinfectants against common bacterial species.
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